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Product Name
ATP1A2 antibody
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Description
ATP1A2 Rabbit Polyclonal antibody. Positive IHC detected in human skin tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human testis tissue. Positive IF detected in Hela cells. Positive FC detected in HeLa cells. Positive WB detected in HEK-293 cells. Observed molecular weight by Western-blot: 97-100 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
alpha 2 ATPase antibody; ATP1A2 antibody; FHM2 antibody; KIAA0778 antibody; MHP2 antibody; Sodium pump subunit alpha 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ATP1A2 recombinant protein (Accession Number: NM_000702). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108342(ATP1A2 antibody) at dilution of 1:1200
Immunohistochemical of paraffin-embedded human skin using Catalog No:108342(ATP1A2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin using Catalog No:108342(ATP1A2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using ATP1A2 antibody Catalog No:108342 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HeLa cells were stained with 0.5ug ATP1A2 antibody (Catalog No:108342, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.
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Background
ATP1A2 (Na+/K+-ATPase α-2 subunit) is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody was raised against the internal region of the human ATP1A2 and can recognizes all the isoforms of α subunit. The 65kDa band detected occasionally may be the degradation product of ATP1A2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"