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Product Name
ATP1A1-Specific antibody
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Description
ATP1A1-Specific Rabbit Polyclonal antibody. Positive FC detected in HEK-293 cells. Positive IF detected in HEK-293 cells. Positive IHC detected in human kidney tissue. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells, MCF7 cells, mouse heart tissue, Neuro-2a cells. Observed molecular weight by Western-blot: 113kd
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Tested applications
ELISA, WB, IHC, FC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ATP1A1 antibody; Na(+)/K(+) ATPase 1 antibody; Na(+)/K(+) ATPase alpha 1 subunit antibody; Na+/K+ ATPase 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_000701). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108341(ATP1A1 antibody) at dilution of 1:2000
Immunofluorescent analysis of HEK-293 cells using Catalog No:108341(ATP1A1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:108341(ATP1A1 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:108341(ATP1A1 antibody) at dilution of 1:100 (under 10x lens)
1X10^6 HEK-293 cells were stained with 0.2ug ATP1A1 antibody (Catalog No:108341, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
ATP1A1 is the catalytic component of Na+/K+-ATPase which is is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.
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