ATP1A1 antibody

Cat.#: 108340

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Product Information

  • Product Name
    ATP1A1 antibody
  • Documents
  • Description
    ATP1A1 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in mouse heart tissue, A549 cells, COLO 320 cells, HEK-293 cells, human colon tissue. Positive IHC detected in human liver cancer tissue, human ovary tumor tissue. Observed molecular weight by Western-blot: 100kd
  • Tested applications
    ELISA, IP, IHC, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ATP1A1 antibody; Na(+)/K(+) ATPase 1 antibody; Na(+)/K(+) ATPase alpha 1 subunit antibody; Na+/K+ ATPase 1 antibody; Sodium potassium ATPase alpha 1 polypeptide antibody; Sodium pump subunit alpha 1 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ATP1A1 recombinant protein (Accession Number: NM_000701). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:117301(ATP1A1 Antibody) at dilution of 1:1000

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:117301(ATP1A1 Antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:117301(ATP1A1 Antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:117301(ATP1A1 Antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:117301(ATP1A1 Antibody) at dilution of 1:200 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:117301(ATP1A1 Antibody) at dilution of 1:200 (under 40x lens)

    IP Result of anti-ATP1A1 (IP:Catalog No:117301, 4ug; Detection:Catalog No:117301 1:300) with HEK-293 cells lysate 2800ug.

    IP Result of anti-ATP1A1 (IP:Catalog No:117301, 4ug; Detection:Catalog No:117301 1:300) with HEK-293 cells lysate 2800ug.

  • Background
    ATP1A1 is the catalytic component of Na+/K+-ATPase which is is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein.
  • References
    • Lai G, Wu J, Liu X, Zhao Y. 20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway. Molecular endocrinology (Baltimore, Md.). 26(11):1907-16. 2012.
    • Xiong Q, Cao Q, Zhou Q. Arrhythmogenic cardiomyopathy in a patient with a rare loss-of-function KCNQ1 mutation. Journal of the American Heart Association. 4(1):e001526. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"