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Product Name
ATP1A Polyclonal Antibody
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Description
Polyclonal antibody to ATP1A
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Tested applications
WB, IHC, IP
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Species reactivity
Human
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Alternative names
ATP1A1 antibody; ATPase Na+/K+ transporting subunit alpha 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 551-850 of human ATP1A (NP_000692.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IP 1:50 - 1:100 -
Validations
Western blot - ATP1A Polyclonal Antibody
Western blot analysis of extracts of LO2 cells, using ATP1A1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - ATP1A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using ATP1A1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ATP1A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using ATP1A1 antibody at dilution of 1:100 (40x lens).
Immunoprecipitation - ATP1A Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of LO2 cells, using 3 ug ATP1A1 antibody . Western blot was performed from the immunoprecipitate using ATP1A1 antibody at a dilition of 1:1000.
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Background
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"