ATP1A Polyclonal Antibody

Cat.#: 160124

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Product Information

  • Product Name
    ATP1A Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to ATP1A
  • Tested applications
    WB, IHC, IP
  • Species reactivity
    Human
  • Alternative names
    ATP1A1 antibody; ATPase Na+/K+ transporting subunit alpha 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 551-850 of human ATP1A (NP_000692.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:1000
    IHC 1:50 - 1:200
    IP 1:50 - 1:100
  • Validations

    Western blot - ATP1A Polyclonal Antibody

    Western blot - ATP1A Polyclonal Antibody

    Western blot analysis of extracts of LO2 cells, using ATP1A1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

    Immunohistochemistry - ATP1A Polyclonal Antibody

    Immunohistochemistry - ATP1A Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human prostate using ATP1A1 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - ATP1A Polyclonal Antibody

    Immunohistochemistry - ATP1A Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human stomach using ATP1A1 antibody at dilution of 1:100 (40x lens).

    Immunoprecipitation - ATP1A Polyclonal Antibody

    Immunoprecipitation - ATP1A Polyclonal Antibody

    Immunoprecipitation analysis of 200ug extracts of LO2 cells, using 3 ug ATP1A1 antibody . Western blot was performed from the immunoprecipitate using ATP1A1 antibody at a dilition of 1:1000.

  • Background
    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"