-
Product Name
ATG13 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to ATG13
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
ATG13 antibody; KIAA0652 antibody; PARATARG8 antibody; autophagy related 13 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 281-480 of human ATG13 (NP_055556.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - ATG13 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ATG13 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 10s.
Immunohistochemistry - ATG13 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat testis using ATG13 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ATG13 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using ATG13 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - ATG13 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using ATG13 antibody . Blue: DAPI for nuclear staining.
-
Background
Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"