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Product Name
ATAD2 antibody
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Description
ATAD2 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 159 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
ANCCA antibody; ATAD2 antibody; DKFZp667N1320 antibody; PRO2000 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ATAD2 recombinant protein (Accession Number: NM_014109). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108277(ATAD2 Antibody) at dilution of 1:600
IP Result of anti-ATAD2 (IP:Catalog No:108277, 4ug; Detection:Catalog No:108277 1:500) with HeLa cells lysate 3200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:108277(ATAD2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ATAD2 is one of them encoding a conserved factor harbouring an AAA type ATPase domain and a bromodomain. ATAD2 controls chromatin dynamics, genome transcriptional activities and apoptotic cell response. This protein has 2 isoforms produced by alternative splicing.
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