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Product Name
ASS1 antibody
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Description
ASS1 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human liver tissue, human placenta tissue. Positive WB detected in HEK-293 cells, HeLa cells, human kidney tissue, mouse kidney tissue, rat kidney tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 45 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Argininosuccinate synthase antibody; argininosuccinate synthetase 1 antibody; ASS antibody; ASS1 antibody; Citrulline aspartate ligase antibody; CTLN1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ASS1 recombinant protein (Accession Number: NM_054012). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108271(ASS1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:108271(ASS1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:108271(ASS1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ASS1 (IP:Catalog No:108271, 4ug; Detection:Catalog No:108271 1:1500) with HeLa cells lysate 1600ug.
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Background
ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).
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References
- Tan GS, Lim KH, Tan HT. Novel proteomic biomarker panel for prediction of aggressive metastatic hepatocellular carcinoma relapse in surgically resectable patients. Journal of proteome research. 13(11):4833-46. 2014.
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