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Product Name
ASRGL1 antibody
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Description
ASRGL1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ALP antibody; ALP1 antibody; asparaginase like 1 antibody; Asparaginase like protein 1 antibody; ASRGL1 antibody; CRASH antibody; FLJ22316 antibody; L asparaginase antibody; L asparagine amidohydrolase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ASRGL1 recombinant protein (Accession Number: NM_001083926). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108270(ASRGL1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:108270(ASRGL1 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-ASRGL1 (IP:Catalog No:108270, 4ug; Detection:Catalog No:108270 1:500) with HeLa cells lysate 3200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:108270(ASRGL1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Asparaginase-like protein 1 (ASRGL1) is also named as ALP, CRASH and belongs to the Ntn-hydrolase family. ASRGL1 has both L-asparaginase and beta-aspartyl peptidase activity. It may be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. ASRGL1 has 2 isoforms produced by alternative splicing with the MW of 32 kDa and 19 kDa.
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References
- Yuan ZS, Zhou YZ, Liao XB. Apelin attenuates the osteoblastic differentiation of aortic valve interstitial cells via the ERK and PI3-K/Akt pathways. Amino acids. 47(12):2475-82. 2015.
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