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ASNS antibody

Cat.#: 108234

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Special Price 239.0 USD

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Product Information

  • Product Name
    ASNS antibody
  • Documents
  • Description
    ASNS Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HeLa cells. Positive IP detected in HeLa cells. Positive IF detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 55-64 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ASNS antibody; asparagine synthetase antibody; TS11 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ASNS recombinant protein (Accession Number: NM_133436). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IF: 1:20-1:200

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:108234(ASNS antibody) at dilution of 1:500

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:108234(ASNS antibody) at dilution of 1:500

    IP Result of anti-ASNS (IP:Catalog No:108234, 4ug; Detection:Catalog No:108234 1:500) with HeLa cells lysate 1200ug.

    IP Result of anti-ASNS (IP:Catalog No:108234, 4ug; Detection:Catalog No:108234 1:500) with HeLa cells lysate 1200ug.

    Immunofluorescent analysis of (-20oc Ethanol) fixed SH-SY5Y cells using Catalog No:108234(ASNS Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed SH-SY5Y cells using Catalog No:108234(ASNS Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). It encodes a protein of about 561 amino acids with the predicted molecular mass of 64 kDa.
  • References
    • Yang H, He X, Zheng Y. Down-regulation of asparagine synthetase induces cell cycle arrest and inhibits cell proliferation of breast cancer. Chemical biology & drug design. 84(5):578-84. 2014.
    • Li H, Zhou F, Du W. Knockdown of asparagine synthetase by RNAi suppresses cell growth in human melanoma cells and epidermoid carcinoma cells. Biotechnology and applied biochemistry. 2015.
    • Zhang J, Fan J, Venneti S. Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion. Molecular cell. 56(2):205-18. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"