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Product Name
ASGR1 antibody
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Description
ASGR1 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in human liver tissue, mouse liver tissue. Positive FC detected in HepG2 cells. Positive IHC detected in human liver cancer tissue, human liver tissue. Observed molecular weight by Western-blot: 40 kDa
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Tested applications
ELISA, WB, IHC, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ASGP R 1 antibody; ASGPR antibody; ASGPR 1 antibody; ASGR1 antibody; asialoglycoprotein receptor 1 antibody; CLEC4H1 antibody; Hepatic lectin H1 antibody; HL 1 antibody; Hs.12056 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ASGR1 recombinant protein (Accession Number: NM_001671). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:108226(ASGR1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:108226(ASGR1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:108226(ASGR1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ASGR1 (IP:Catalog No:108226, 4ug; Detection:Catalog No:108226 1:500) with mouse liver tissue lysate 6400ug.
1X10^6 HepG2 cells were stained with 0.2ug ASGR1 antibody (Catalog No:108226, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.
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