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Product Name
Anti-ASGPR1 antibody
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Description
Rabbit polyclonal to ASGPR1
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Mouse ASGPR1 / ASGR1
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Alternative names
mCG_22510 antibody; Asgr-1 antibody; Asialoglycoprotein receptor 1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse ASGPR1 / ASGR1 (rM ASGPR1 / ASGR1; NP_033844.1; Ser 60-Asn 284). ASGPR1 / ASGR1 specific IgG was purified by Mouse ASGPR1 / ASGR1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-50 μg/mL
ELISA: 0.5-1.0 μg/mL
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Mouse ASGR1. The detection limit for Mouse ASGR1 is 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
ASGPR1 / ASGR1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
ASGPR1 / ASGR1 Antibody, Rabbit PAb, Antigen Affinity Purified
ASGPR1 / ASGR1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse ASGR1 in mouse liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development.
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References
- Yang J, et al. (2006) Antisense oligonucleotides targeted against asialoglycoprotein receptor 1 block human hepatitis B virus replication. J Viral Hepat. 13(3): 158-65.
- Li Y, et al. (2008) Targeted delivery of macromolecular drugs: asialoglycoprotein receptor (ASGPR) expression by selected hepatoma cell lines used in antiviral drug development. Curr Drug Deliv. 5(4): 299-302.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"