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Product Name
ASF/SF2 antibody
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Description
ASF/SF2 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, HeLa cells, human ileum tissue, human placenta tissue, mouse brain tissue. Observed molecular weight by Western-blot: 28kd
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Alternative splicing factor 1 antibody; ASF antibody; ASF 1 antibody; ASF/SF2 antibody; SF2 antibody; SF2p33 antibody; SFRS1 antibody; SRp30a antibody; SRSF1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ASF/SF2 recombinant protein (Accession Number: XM_047436526). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108221(ASF/SF2 antibody) at dilution of 1:1000
IP Result of anti-ASF/SF2 (IP:Catalog No:108221, 3ug; Detection:Catalog No:108221 1:500) with mouse brain tissue lysate 3600ug.
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Background
SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.
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References
- Huang C, Cao W, Liao R. PP1γ functionally augments the alternative splicing of CaMKIIδ through interaction with ASF. American journal of physiology. Cell physiology. 306(2):C167-77. 2014.
- Liao RJ, Tong LJ, Huang C. Rescue of cardiac failing and remodelling by inhibition of protein phosphatase 1γ is associated with suppression of the alternative splicing factor-mediated splicing of Ca2+/calmodulin-dependent protein kinase δ. Clinical and experimental pharmacology & physiology. 41(12):976-85. 2014.
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