ASF/SF2 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, HeLa cells, human ileum tissue, human placenta tissue, mouse brain tissue. Observed molecular weight by Western-blot: 28kd
ELISA, WB, IP
Human,Mouse,Rat; other species not tested.
Alternative splicing factor 1 antibody; ASF antibody; ASF 1 antibody; ASF/SF2 antibody; SF2 antibody; SF2p33 antibody; SFRS1 antibody; SRp30a antibody; SRSF1 antibody
This antibody was obtained by immunization of ASF/SF2 recombinant protein (Accession Number: BC010264). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108221(ASF/SF2 antibody) at dilution of 1:1000
IP Result of anti-ASF/SF2 (IP:Catalog No:108221, 3ug; Detection:Catalog No:108221 1:500) with mouse brain tissue lysate 3600ug.
SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.
- Huang C, Cao W, Liao R. PP1γ functionally augments the alternative splicing of CaMKIIδ through interaction with ASF. American journal of physiology. Cell physiology. 306(2):C167-77. 2014.
- Liao RJ, Tong LJ, Huang C. Rescue of cardiac failing and remodelling by inhibition of protein phosphatase 1γ is associated with suppression of the alternative splicing factor-mediated splicing of Ca2+/calmodulin-dependent protein kinase δ. Clinical and experimental pharmacology & physiology. 41(12):976-85. 2014.
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