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Product Name
ARPC5L antibody
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Description
ARPC5L Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IHC detected in human placenta tissue, human cerebellum tissue. Positive IF detected in MCF-7 cells. Positive WB detected in mouse brain tissue, human brain tissue, human placenta tissue, mouse lung tissue, NIH/3T3 cells. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 17-20 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARC16 2 antibody; ARPC5L antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARPC5L recombinant protein (Accession Number: NM_030978). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108169(ARPC5L antibody) at dilution of 1:1000
Immunofluorescent analysis of MCF-7 cells using Catalog No:108169(ARPC5L Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Immunohistochemical of paraffin-embedded human placenta using Catalog No:108169(ARPC5L antibody) at dilution of 1:50 (under 10x lens)
1X10^6 MCF-7 cells were stained with 0.2ug ARPC5L antibody (Catalog No:108169, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
IP Result of anti-ARPC5L (IP:Catalog No:108169, 4ug; Detection:Catalog No:108169 1:1000) with mouse brain tissue lysate 4000ug.
Immunohistochemical of paraffin-embedded human placenta using Catalog No:108169(ARPC5L antibody) at dilution of 1:50 (under 40x lens)
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Background
ARPC5L represents actin related protein 2/3 complex, subunit 5-like
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