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Product Name
Arp5/ACTR5 antibody
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Description
Arp5/ACTR5 Rabbit Polyclonal antibody. Positive IF detected in A549 cells, HepG2 cells. Positive IP detected in HEK-293 cells. Positive WB detected in mouse cerebellum tissue, HEK-293 cells, human skeletal muscle tissue, mouse heart tissue, mouse ovary tissue. Observed molecular weight by Western-blot: 68 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Actin related protein 5 antibody; ACTR5 antibody; Arp5 antibody; hARP5 antibody; Sarcoma antigen NY SAR 16 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Arp5/ACTR5 recombinant protein (Accession Number: NM_024855). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with Catalog No:108163(ACTR5 antibody) at dilution of 1:500
IP Result of anti-ACTR5 (IP:Catalog No:108163, 4ug; Detection:Catalog No:108163 1:500) with HEK-293 cells lysate 1120ug.
Immunofluorescent analysis of A549 cells using Catalog No:108163(ACTR5 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)
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References
- Morita T, Hayashi K. Arp5 is a key regulator of myocardin in smooth muscle cells. The Journal of cell biology. 204(5):683-96. 2014.
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