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Product Name
ARL5B antibody
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Description
ARL5B Rabbit Polyclonal antibody. Positive WB detected in human liver tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 25 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ARL5B antibody; ARL8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARL5B recombinant protein (Accession Number: NM_178815). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:108144(ARL5B antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:108144(ARL5B antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using ARL5B antibody Catalog No:108144 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
ARL5B represents ADP-ribosylation factor-like 5B
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References
- Kitai Y, Takeuchi O, Kawasaki T. Negative regulation of melanoma differentiation-associated gene 5 (MDA5)-dependent antiviral innate immune responses by Arf-like protein 5B. The Journal of biological chemistry. 290(2):1269-80. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"