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Product Name
ARHGEF9 Polyclonal Antibody
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Description
Polyclonal antibody to ARHGEF9
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ARHGEF9 antibody; COLLYBISTIN antibody; EIEE8 antibody; HPEM-2 antibody; PEM-2 antibody; PEM2 antibody; rho guanine nucleotide exchange factor 9 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 307-516 of human ARHGEF9 (NP_056000.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - ARHGEF9 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ARHGEF9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - ARHGEF9 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using ARHGEF9 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - ARHGEF9 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using ARHGEF9 antibody . Blue: DAPI for nuclear staining.
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Background
Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"