-
Product Name
ARHGEF5 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to ARHGEF5
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
ARHGEF5 antibody; GEF5 antibody; P60 antibody; TIM antibody; TIM1 antibody; rho guanine nucleotide exchange factor 5 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1248-1597 of human ARHGEF5 (NP_005426.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200 -
Validations
Western blot - ARHGEF5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ARHGEF5 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - ARHGEF5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using ARHGEF5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ARHGEF5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using ARHGEF5 antibody at dilution of 1:100 (40x lens).
-
Background
Guanine nucleotide exchange factor which activates Rho GTPases. Strongly activates RHOA. Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity). Involved in regulation of cell shape and actin cytoskeletal organization. Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia. Required for SRC-induced podosome formation (By similarity). Involved in positive regulation of immature dendritic cell migration (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"