ARHGEF5 Polyclonal Antibody

Cat.#: 164282

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Product Information

  • Product Name
    ARHGEF5 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to ARHGEF5
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ARHGEF5 antibody; GEF5 antibody; P60 antibody; TIM antibody; TIM1 antibody; rho guanine nucleotide exchange factor 5 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1248-1597 of human ARHGEF5 (NP_005426.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IF 1:50 - 1:200
  • Validations

    Western blot - ARHGEF5 Polyclonal Antibody

    Western blot - ARHGEF5 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using ARHGEF5 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunohistochemistry - ARHGEF5 Polyclonal Antibody

    Immunohistochemistry - ARHGEF5 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse liver using ARHGEF5 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - ARHGEF5 Polyclonal Antibody

    Immunohistochemistry - ARHGEF5 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse kidney using ARHGEF5 antibody at dilution of 1:100 (40x lens).

  • Background
    Guanine nucleotide exchange factor which activates Rho GTPases. Strongly activates RHOA. Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity). Involved in regulation of cell shape and actin cytoskeletal organization. Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia. Required for SRC-induced podosome formation (By similarity). Involved in positive regulation of immature dendritic cell migration (By similarity).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"