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Product Name
ARF3 antibody
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Description
ARF3 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, HeLa cells, HepG2 cells, human brain tissue, mouse cerebellum tissue, Raji cells, rat brain tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas tissue, human pancreas cancer tissue. Observed molecular weight by Western-blot: 18-20 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
ADP ribosylation factor 3 antibody; ARF3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARF3 recombinant protein (Accession Number: XM_005268856). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108245(ARF3 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:108245(ARF3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:108245(ARF3 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:108245(ARF3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF3 is 95% homolouous to ARF1. Its function was not fully understood. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.
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