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Product Name
Anti-ARF3 / ADP-ribosylation factor 3 antibody
- Documents
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Description
Rabbit polyclonal to ARF3 / ADP-ribosylation factor 3
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Tested applications
ELISA, WB, IF, ICC/IF
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Species reactivity
Human ARF3 / ADP-ribosylation factor 3
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Alternative names
ADP-ribosylation factor 3 antibody; ARF3 antibody; small GTP binding protein antibody; ADP-ribosylation factor 3 antibody; Arf3 antibody; 5430400P17Rik antibody; AI854770 antibody; AI854770 antibody; 5430400P17Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human ARF3 / ADP-ribosylation factor 3 (rh ARF3 / ADP-ribosylation factor 3; P61204; Met1-Lys181). ARF3 / ADP-ribosylation factor 3 specific IgG was purified by Human ARF3 / ADP-ribosylation factor 3 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ARF3 / ADP-ribosylation factor 3.
ICC/IF: 0.5-1.5 μg/mL
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Validations
ARF3 / ADP-ribosylation factor 3 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
ARF3 / ADP-ribosylation factor 3 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of ARF3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ARF3 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
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Background
ARF3, also known as ADP-ribosylation factor 3, belongs to the RAS superfamily. Members of this family include ARF1, ARF2, ARF3, ARF4, ARF5 and ARF6. ARF3 gene is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. ARF3 functions as an allosteric activator of the cholera toxin subunit, an ADP-ribosyltransferase. It is involved in protein trafficking and may modulate vesicle budding and uncoating within the Golgi apparatus.
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References
- Hirai M. et al., 1997, Genomics. 34 (2): 263-5.
- Kanoh H. et al., 1997, J Biol Chem. 272 (9): 5421-9.
- Boman. et al., 2002, Mol Biol Cell. 13 (9): 3078-95.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"