AQP2 Polyclonal Antibody

Cat.#: 163089

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Product Information

  • Product Name
    AQP2 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to AQP2
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    AQP2 antibody; AQP-CD antibody; WCH-CD antibody; aquaporin-2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 147-271 of human AQP2 (NP_000477.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IF 1:50 - 1:200
  • Validations

    Western blot - AQP2 Polyclonal Antibody

    Western blot - AQP2 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using AQP2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

    Immunohistochemistry - AQP2 Polyclonal Antibody

    Immunohistochemistry - AQP2 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse kidney using AQP2 antibody at dilution of 1:100 (40x lens).

    Immunofluorescence - AQP2 Polyclonal Antibody

    Immunofluorescence - AQP2 Polyclonal Antibody

    Immunofluorescence analysis of rat kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - AQP2 Polyclonal Antibody

    Immunofluorescence - AQP2 Polyclonal Antibody

    Immunofluorescence analysis of mouse kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - AQP2 Polyclonal Antibody

    Immunofluorescence - AQP2 Polyclonal Antibody

    Immunofluorescence analysis of rat kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - AQP2 Polyclonal Antibody

    Immunofluorescence - AQP2 Polyclonal Antibody

    Immunofluorescence analysis of mouse kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - AQP2 Polyclonal Antibody

    Immunofluorescence - AQP2 Polyclonal Antibody

    Immunofluorescence analysis of rat kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - AQP2 Polyclonal Antibody

    Immunofluorescence - AQP2 Polyclonal Antibody

    Immunofluorescence analysis of mouse kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"