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Product Name
AQP2 Polyclonal Antibody
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Description
Polyclonal antibody to AQP2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
AQP2 antibody; AQP-CD antibody; WCH-CD antibody; aquaporin-2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 147-271 of human AQP2 (NP_000477.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200 -
Validations
Western blot - AQP2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using AQP2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - AQP2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using AQP2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - AQP2 Polyclonal Antibody
Immunofluorescence analysis of rat kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - AQP2 Polyclonal Antibody
Immunofluorescence analysis of mouse kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - AQP2 Polyclonal Antibody
Immunofluorescence analysis of rat kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - AQP2 Polyclonal Antibody
Immunofluorescence analysis of mouse kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - AQP2 Polyclonal Antibody
Immunofluorescence analysis of rat kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - AQP2 Polyclonal Antibody
Immunofluorescence analysis of mouse kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"