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Product Name
APTX Polyclonal Antibody
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Description
Polyclonal antibody to APTX
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Tested applications
WB, IF, IP
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Species reactivity
Human, Mouse
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Alternative names
APTX antibody; AOA antibody; AOA1 antibody; AXA1 antibody; EAOH antibody; EOAHA antibody; FHA-HIT antibody; aprataxin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:20 - 1:50 -
Validations
Western blot - APTX Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using APTX antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - APTX Polyclonal Antibody
Immunofluorescence analysis of A549 cells using APTX antibody .
Immunoprecipitation - APTX Polyclonal Antibody
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug APTX antibody . Western blot was performed from the immunoprecipitate using APTX antibody at a dilition of 1:500.
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Background
DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA) (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"