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Product Name
Apolipoprotein AI antibody
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Description
Apolipoprotein AI Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue, human lung tissue. Positive FC detected in HepG2 cells. Positive WB detected in human blood tissue, human brain tissue, human ileum tissue, mouse lung tissue, PC-13 cells. Positive IP detected in mouse lung tissue. Observed molecular weight by Western-blot: 26-30kd
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Tested applications
ELISA, WB, IHC, FC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Apo AI antibody; ApoA I antibody; APOA1 antibody; apolipoprotein A I antibody; Apolipoprotein A1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Apolipoprotein AI recombinant protein (Accession Number: NM_000039). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human blood tissue were subjected to SDS PAGE followed by western blot with Catalog No:108037(APOA1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:108037(APOA1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:108037(APOA1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-APOA1 (IP:Catalog No:108037, 4ug; Detection:Catalog No:108037 1:500) with mouse lung tissue lysate 2800ug.
1X10^6 HepG2 cells were stained with 0.2ug APOA1 antibody (Catalog No:108037, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.
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References
- Lihong H, Linlin G, Yiping G. Proteomics approaches for identification of tumor relevant protein targets in pulmonary squamous cell carcinoma by 2D-DIGE-MS. PloS one. 9(4):e95121. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"