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Product Name
Anti-ApoA1 antibody
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Description
Rabbit polyclonal to ApoA1
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human APOA1 / ApoAI
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Alternative names
42249 antibody; Alp-1 antibody; Ltw-1 antibody; 42248 antibody; Apoa-1 antibody; Brp-14 antibody; Lvtw-1 antibody; apo-AI antibody; apoA-I antibody; Apoa-1 antibody; Brp-14 antibody; Ltw-1 antibody; Lvtw-1 antibody; MGC102525 antibody; ALP-1 antibody; MGC117399 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human APOA1 / ApoAI (rh APOA1 / ApoAI; P02647; Asp25-Gln267).APOA1 / ApoAI specific IgG was purified by Human APOA1 / ApoAI affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ApoA1. The detection limit for Human ApoA1 is 0.00975 ng/well.
IHC-P: 0.1-1 μg/mL
IP: 0.5-2 μg/mg of lysate
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Validations
APOA1 / ApoAI Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
APOA1 / ApoAI Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
APOA1 / ApoAI Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human APOA1 in human cirrhosis with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Apolipoprotein A1 (APOA1) is a member of the apolipoprotein family whose members are proteins bind with lipids and form lipoproteins to translate these oil-soluble lipids such as fat and cholesterol through lymphatic and circulatory system. APOA1 is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters, and thus play a major role in cholesterol efflux from peripheral cells. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Men carrying a mutation may develop premature coronary artery disease.
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References
- Toptas B, et al. (2011) Comparison of lipid profiles with APOA1 MspI polymorphism in obese children with hyperlipidemia. In Vivo. 25(3): 425-30.
- Haase CL, et al. (2011) Mutation in APOA1 predicts increased risk of ischaemic heart disease and total mortality without low HDL cholesterol levels. J Intern Med. 270(2): 136-46.
- Wu Z, et al. (2011) The low resolution structure of ApoA1 in spherical high density lipoprotein revealed small angle neutron scattering. J Biol Chem. 286(14): 12495-508.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"