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Product Name
Mouse ApoA1 (Fc Tag) recombinant protein
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Description
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
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Protein name
Apolipoprotein A-I
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Protein short names
APO-AI; BRP-14; ALP-1; MGC102525; LTW-1; APOA-I; MGC117399; APOA-1; 42249; 42248; LVTW-1
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Uniprot ID
Q00623
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Gene Name
Apoa1
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse APOA1 (Q00623) (Met 1-Gln 264) was fused with the Fc region of human IgG1 at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The secreted recombinant mouse APOA1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 487 amino acids and has a calculated molecular mass of 55.8 kDa. As a result of glycosylation, the apparent molecular mass of rmAPOA1/Fc monomer is approximately 60 kDa in SDS-PAGE under reducing conditions.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
1. Measured by its binding ability in a functional ELISA.
2. Immobilized mouse ApoAI at 10 μg/mL (100 μl/well) can bind biotinylated human SCARB1, The EC50 of biotinylated human SCARB1 is 0.27 μg/mL. -
Validations
Mouse APOA1 Protein (Fc Tag) SDS-PAGE
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