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Product Name
APOBEC3B antibody
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Description
APOBEC3B Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in MDA-MB-453s cells, Transfected HEK-293 cells. Positive IP detected in mouse spleen tissue. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, IP, IHC, WB
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Species reactivity
Human; other species not tested.
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Alternative names
APOBEC1L antibody; APOBEC3B antibody; ARCD3 antibody; ARP4 antibody; bK150C2.2 antibody; DJ742C19.2 antibody; FLJ21201 antibody; Phorbolin 1 related protein antibody; Phorbolin 2/3 antibody; PHRBNL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of APOBEC3B recombinant protein (Accession Number: BC053859). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with Catalog No:108025(APOBEC3B Antibody) at dilution of 1:300
IP Result of anti-APOBEC3B (IP:Catalog No:108025, 4ug; Detection:Catalog No:108025 1:500) with mouse spleen tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:108025(APOBEC3B Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
The DNA cytosine deaminase APOBEC3B was identified recently as a source of DNA damage and mutagenesis in breast, head/neck, cervix, bladder, lung, ovary, and to lesser extents additional cancer types. This enzyme is also an effector protein in the innate immune response to virus infection. APOBEC3B has 3 isoforms produced by alternative splicing with the MW of 46, 29, 43 kDa. It can also exsit as a dimer and can interact with APOBEC3G.
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