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Product Name
APOB antibody
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Description
APOB Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, HEK-293 cells, HepG2 cells, L02 cells. Positive IHC detected in human liver tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 130-150 kDa, 200-250 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Apo B 100 antibody; Apo B 48 antibody; APOB antibody; ApoB100 antibody; Apolipoprotein B antibody; Apolipoprotein B 100 antibody; FLDB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_000384). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemistry of paraffin-embedded human liver slide using Catalog No:108023(APOB Antibody) at dilution of 1:50
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:108023(APOB Antibody) at dilution of 1:600
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:108023(APOB Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The apolipoprotein B (ApoB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The ApoB gene encodes two different isoproteins through mRNA editing, ApoB-48 and ApoB-100. ApoB-100 is synthesized exclusively by the liver and is essential for the assembly of VLDL in the liver. ApoB-48 is synthesized exclusively by the small intestine and is essential for the assembly of chylomicrons in the intestine. ApoB-48 and ApoB-100 share a common N-terminal sequence, but ApoB-48 lacks ApoB-100's C-terminal LDL receptor binding region. It is well established that ApoB-100 levels are associated with coronary heart disease. This antibody recognizes both of ApoB-48 and ApoB-100.
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