APEX1 antibody

APEX1 antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

APEX1 Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, HEK-293 cells, K-562 cells, mouse liver tissue, Raji cells. Observed molecular weight by Western-blot: 36 kDa

ELISA, WB, IHC, IF

Human,Mouse,Rat; other species not tested.

AP endonuclease 1 antibody; APE antibody; APE1 antibody; APE1 antibody; APEX1 antibody; APEN antibody; APEX antibody; APEX nuclease antibody; APEX1 antibody; APX antibody; HAP1 antibody; Protein REF 1 antibody; REF1 antibody

Rabbit IgG

This antibody was obtained by immunization of APEX1 recombinant protein (Accession Number: NM_001244249). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression. 10323-1-AP is a rabbit polyclonal antibody raised against a fusion protein corresponding to an internal region of human APEX1.