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Product Name
AP3M1 antibody
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Description
AP3M1 Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells. Positive IP detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 47 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AP 3 complex subunit mu 1 antibody; AP3M1 antibody; Mu adaptin 3A antibody; Mu3A adaptin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AP3M1 recombinant protein (Accession Number: XM_024447939). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:108124(AP3M1 antibody) at dilution of 1:400
IP Result of anti-AP3M1 (IP:Catalog No:108124, 4ug; Detection:Catalog No:108124 1:300) with NIH/3T3 cells lysate 1200ug.
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Background
Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
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References
- Guo Y, Zanetti G, Schekman R. A novel GTP-binding protein-adaptor protein complex responsible for export of Vangl2 from the trans Golgi network. eLife. 2:e00160. 2013.
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