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Product Name
Anti-YBX2 antibody
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Description
Rabbit polyclonal antibody to YBX2
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Tested applications
WB, ICC, IHC-P, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
DBPC antibody; MSY2 antibody; CSDA3 antibody; CONTRIN antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was synthetic peptide within human ybox1 aa 302-355.
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Validations
Fig1: Western blot analysis on mouse testis lysates using anti-YB1 rabbit polyclonal antibody.
Fig2: Immunocytochemical staining of Hela cells using anti-YB1 rabbit polyclonal antibody.
Fig3: Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-YB1 rabbit polyclonal antibody.
Fig4: Immunohistochemical analysis of paraffin- embedded mouse ovary tissue using anti-YB1 rabbit polyclonal antibody.
Fig5: Flow cytometric analysis of Hela cells with YB1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
- Background
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References
- Chalmey, C. et al. 2013. Systemic compensatory response to neonatal estradiol exposure does not prevent depletion of the oocyte pool in the rat. PloS one. 8: e82175.
- Shi, K. et al. 2007. Advanced methods of isolation and identification of porcine primordial follicles. Anim Reprod Sci. 101: 163-171.
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