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Product Name
Anti-VWF antibody
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Description
Mouse monoclonal to VWF
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Tested applications
ELISA, IHC-P
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Species reactivity
Human vWF
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Alternative names
VWD antibody; F8VWF antibody; VWD antibody; F8VWF antibody; AI551257 antibody; C630030D09 antibody; 6820430P06Rik antibody; B130011O06Rik antibody; 6820430P06Rik antibody; AI551257 antibody; B130011O06Rik antibody; C630030D09 antibody; F8VWF antibody; F8VWF antibody; VWD antibody; VWD antibody; Vwf antibody; VWF antibody
- Immunogen
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Isotype
Mouse IgG2a
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human von Willebrand factor (rhvWF; aa 1-2813; NP_000543.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.25-0.5 μg/mL
This antibody can be used at 0.25-0.5 μg/mL with the appropriate secondary reagents to detect vWF. The detection limit for vWF is approximately 10 ng/well.
IHC-P: 1-5 μg/mL
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Validations
vWF Antibody, Mouse MAb, Immunohistochemistry
Immunochemical staining of human vWF in human stomach with mouse monoclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to blood vessel.
vWF Antibody, Mouse MAb, Immunohistochemistry
Immunochemical staining of human vWF in human tonsil with mouse monoclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to blood vessel.
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Background
Von Willebrand Factor (VWF) is a multimeric glycoprotein involved in hemostasis in blood, binds receptors on the surface of platelets and in connective tissue, thereby mediating the adhesion of platelets to sites of vascular injury. From studies it appears that VWF protein uncoils under these circumstances, decelerating passing platelets. VWF protein is deficient or defective in von Willebrand disease (VWD) and is involved in a large number of other diseases, including thrombosis, thrombotic thrombocytopenic purpura, Stroke, Heyde's syndrome, possibly hemolytic-uremic syndrome and so on.
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References
- Sadler JE. (1998) Biochemistry and genetics of von Willebrand factor. Annu Rev Biochem, 67: 395-424.
- Batlle J, et al. (2009) Von Willebrand factor/factor VIII concentrates in the treatment of von Willebrand disease. Blood Coagul Fibrinolysis. 20(2):89-100.
- Sadler JE. (2009) von Willebrand factor assembly and secretion. J Thromb Haemost. 7 Suppl 1:24-7.
- Auton M, et al. (2010) The mechanism of VWF-mediated platelet GPIbalpha binding. Biophys J. 99(4):1192-201.
- Bowen DJ. (2010) Sugar targets VWF for the chop. Blood. 115(13):2565.
- Lpez JA, et al. (2010) VWF self-association: more bands for the buck. Blood. 116(19):3693-4.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"