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Anti-VAX1 antibody

Cat.#: 175745

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-VAX1 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to VAX1
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Mouse, Rat, Chicken, Dog, Pig, Cow
  • Alternative names
    MCOPS11 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from human vax1 133-200/334
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    FC:0.2μg/Test

  • Validations

    Fig1: Sample: RAW264.7 Cell (Mouse) Lysate at 40 ug; Primary: Anti- VAX1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 35 kD; Observed band size: 37 kD

    Fig1: Sample: RAW264.7 Cell (Mouse) Lysate at 40 ug; Primary: Anti- VAX1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 35 kD; Observed band size: 37 kD

    Fig2: Sample: BRL-3A Cell (Rat) Lysate at 40 ug; Primary: Anti- VAX1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 35 kD; Observed band size: 37 kD

    Fig2: Sample: BRL-3A Cell (Rat) Lysate at 40 ug; Primary: Anti- VAX1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 35 kD; Observed band size: 37 kD

    Fig3: Sample: NIH/3T3 Cell (Mouse) Lysate at 40 ug; Primary: Anti- VAX1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 35 kD; Observed band size: 37 kD

    Fig3: Sample: NIH/3T3 Cell (Mouse) Lysate at 40 ug; Primary: Anti- VAX1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 35 kD; Observed band size: 37 kD

    Fig4: Sample:; MCF-7 Cell (Human) Lysate at 40 ug; U937 Cell (Human) Lysate at 40 ug; Primary: Anti-VAX1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 35 kD; Observed band size: 36 kD

    Fig4: Sample:; MCF-7 Cell (Human) Lysate at 40 ug; U937 Cell (Human) Lysate at 40 ug; Primary: Anti-VAX1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 35 kD; Observed band size: 36 kD

    Fig5: Blank control (blue line): Hep G2 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% methanol for 20 min at -20℃).; Primary Antibody (green line): Rabbit Anti-VAX1 antibody ,Dilution: 0.2μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

    Fig5: Blank control (blue line): Hep G2 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% methanol for 20 min at -20℃).; Primary Antibody (green line): Rabbit Anti-VAX1 antibody ,Dilution: 0.2μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

  • Background
    Q5SQQ9

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"