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Product Name
Anti-TXNRD1 antibody
- Documents
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Description
Mouse monoclonal to TXNRD1
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Tested applications
FCM, IF, ICC/IF
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Species reactivity
Human TXNRD1
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Alternative names
Tr antibody; TR1 antibody; TR antibody; GRIM-12 antibody; MGC9145 antibody; TR antibody; TR1 antibody; TRXR1 antibody; TrxR1 antibody; TXNR antibody; TXNRD1 antibody; Txnrd1 antibody; Tr1 antibody; TR antibody; TR1 antibody; TXNR antibody; TRXR1 antibody; GRIM-12 antibody; TrxR1 antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TXNRD1 (rh TXNRD1; Q16881-1; Tyr161-Cys647). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
FCM: 0.5-2 μg/Test
ICC/IF: 10-25 μg/mL
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Validations
TXNRD1 Antibody, Mouse MAb
Flow cytometric analysis of Human TXNRD1 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human C1QBP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
TXNRD1 Antibody, Mouse MAb, Immunofluorescence
Immunofluorescence staining of Human TXNRD1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human TXNRD1 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.
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References
- Gasdaska P.Y., et al., 1995, FEBS Lett. 373:5-9.
- Rundloef A.-K., et al., 2004, Free Radic. Biol. Med. 36:641-656.
- Rikova K., et al., 2007, Cell 131:1190-1203.
- Fritz-Wolf K., et al., 2007, J. Mol. Biol. 370:116-127.
- Choudhary C., et al., 2009, Science 325:834-840.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"