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Product Name
Anti-TUBE1 antibody
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Description
Mouse monoclonal antibody to TUBE1
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human
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Alternative names
TUBE antibody; dJ142L7.2 antibody
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Isotype
Mouse IgG1
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Monoclonal
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Formulation
Liquid, 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:2,000
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Validations
Fig1: Western blot analysis of TUBE1 on human TUBE1 recombinant protein using anti-TUBE1 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of TUBE1 on HEK293 (1) and TUBE1-hIgGFc transfected HEK293 (2) cell lysate using anti-TUBE1 antibody at 1/1,000 dilution.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TUBE1 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TUBE1 antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of Hela cells with TUBE1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
- Background
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References
- Chang P et al. Epsilon-tubulin is required for centriole duplication and microtubule organization. Nat Cell Biol. 2003 Jan;5(1):71-6.
- Chang P et al. Delta-tubulin and epsilon-tubulin: two new human centrosomal tubulins reveal new aspects of centrosome structure and function. Nat Cell Biol. 2000 Jan;2(1):30-5.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"