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Anti-TUBB1 antibody

Cat.#: 175095

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-TUBB1 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to TUBB1
  • Tested applications
    WB, IHC-P, FC
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Mouse IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from human beta i tubulin
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:5000-20000

    IHC-P:1:400-800

    FC:1:100-500

  • Validations

    Fig1: Sample:; K562 Cell (Human) Lysate at 40 ug; Jurkat Cell (Human) Lysate at 40 ug; Primary: Anti- beta I Tubulin at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution; Predicted band size: 50 kD; Observed band size: 50 kD

    Fig1: Sample:; K562 Cell (Human) Lysate at 40 ug; Jurkat Cell (Human) Lysate at 40 ug; Primary: Anti- beta I Tubulin at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution; Predicted band size: 50 kD; Observed band size: 50 kD

    Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

    Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

    Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (175095# 5F7) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

    Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (175095# 5F7) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

  • Background
    Q9H4B7

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"