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Anti-THRA antibody

Cat.#: 175724

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-THRA antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to THRA
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Mouse, Rat, Chicken, Pig, Horse, Rabbit, Sheep, Guinea Pig
  • Alternative names
    AR7 antibody; EAR7 antibody; ERBA antibody; CHNG6 antibody; ERBA1 antibody; NR1A1 antibody; THRA1 antibody; THRA2 antibody; ERB-T-1 antibody; TRalpha antibody; c-ERBA-1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from human thyroid hormone receptor alpha 81-180/490
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    FC:1ug/Test

  • Validations

    Fig1: Sample:; Liver (Rat) Lysate at 40 ug; Primary: Anti- Thyroid hormone receptor alpha at 1/500 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 55 kD

    Fig1: Sample:; Liver (Rat) Lysate at 40 ug; Primary: Anti- Thyroid hormone receptor alpha at 1/500 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 55 kD

    Fig2: Sample:; Liver (Mouse) Lysate at 40 ug; Primary: Anti-Thyroid hormone receptor alpha at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 55 kD

    Fig2: Sample:; Liver (Mouse) Lysate at 40 ug; Primary: Anti-Thyroid hormone receptor alpha at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 55 kD

    Fig3: Blank control(blue): Hela(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).; Primary Antibody:Rabbit Anti- Thyroid hormone receptor alpha antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

    Fig3: Blank control(blue): Hela(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).; Primary Antibody:Rabbit Anti- Thyroid hormone receptor alpha antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

    Fig4: Sample:; Liver (Mouse) Lysate at 40 ug; Primary: Anti- Thyroid hormone receptor alpha at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 55 kD

    Fig4: Sample:; Liver (Mouse) Lysate at 40 ug; Primary: Anti- Thyroid hormone receptor alpha at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 55 kD

  • Background
    P10827

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"