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Product Name
Anti-TESPA1 antibody
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Description
Rabbit polyclonal antibody to TESPA1
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Tested applications
WB, ICC, IHC-P, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
HSPC257 antibody; ITPRID3 antibody; KIAA0748 antibody
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Isotype
IgG
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Preparation
This antigen of this antibody was synthetic peptide within human tespa1 aa 226-270.
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:1,000
ICC: 1:200
IHC-P: 1:50
FC: 1:50-1:100
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Validations
Fig1: Western blot analysis of TESPA1 on different lysates using anti-TESPA1 antibody at 1/1,000 dilution.
Fig2: ICC staining TESPA1 in A172 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of HepG2 cells with TESPA1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondar
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