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Product Name
Anti-Syndecan-1/SDC1/CD138 antibody
- Documents
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Description
Rabbit polyclonal to Syndecan-1/SDC1/CD138
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse Syndecan-1 / SDC1 / CD138
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Alternative names
CD138 antibody; CD138 antibody; SDC1 antibody; Sstn antibody; syndecan antibody; Syndecan-1 antibody; Syndecan-1 antibody; SDC antibody; CD138 antibody; SYND1 antibody; syndecan antibody; Sstn antibody; Synd antibody; CD138 antibody; Synd1 antibody; syn-1 antibody; AA408134 antibody; AA409076 antibody; AA408134 antibody; AA409076 antibody; SDC antibody; Sdc1 antibody; syn-1 antibody; Synd antibody; Synd1 antibody; SYND1 antibody; syndecan antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse Syndecan-1 / SDC1 / CD138 (rM Syndecan-1 / SDC1 / CD138; NP_035649.1; Met 1-Glu 252). Syndecan-1 / SDC1 / CD138 specific IgG was purified by Mouse Syndecan-1 / SDC1 / CD138 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse SDC1. The detection limit for Mouse SDC1 is approximately 0.039 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
Syndecan-1 / SDC1 / CD138 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse SDC1 in mouse intestine with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Syndecan-1 / SDC1 / CD138 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse SDC1 in mouse kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
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Background
Syndecan-1 also known as SDC1 and CD138, is the most extensively studied member of the syndecan family. It is found mainly in epithelial cells, but its expression is developmentally regulated during embryonic development. Syndecan-1/SDC1/CD138 has been shown to mediate cell adhesion to several ECM molecules, and to act as a coreceptor for fibroblast growth factors, potent angiogenic growth factors involved also in differentiation. Syndecan-1/SDC1/CD138 expression is reduced during malignant transformation of various epithelia, and this loss correlates with the histological differentiation grade of squamous cell carcinomas, lacking from poorly differentiated tumours. In squamous cell carcinomas of the head and neck, positive syndecan-1 expression correlates with a more favourable prognosis. Experimental studies on the role of Syndecan-1 in malignant transformation have shown that Syndecan-1/SDC1/CD138 expression is associated with the maintenance of epithelial morphology, anchorage-dependent growth and inhibition of invasiveness in vitro.
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References
- Inki P, et al. (1996) The role of syndecan-1 in malignancies. Ann Med. 28(1): 63-7.
- Subramanian SV, et al. (1997) Regulated shedding of syndecan-1 and -4 ectodomains by thrombin and growth factor receptor activation. J Biol Chem. 272(23): 14713-20.
- Park PW, et al. (2001) Exploitation of syndecan-1 shedding by Pseudomonas aeruginosa enhances virulence. Nature. 411(6833): 98-102.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"