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Product Name
Anti-Synaptophysin (10C8) Mouse antibody
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Description
Synaptophysin (10C8) Mouse monoclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human, Rat
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of SYP expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Western blot detection of Synaptophysin in Rat Brain,Rat Hippocampus,Hela,3T3 cell lysates using Synaptophysin (10C8) Mouse mAb(1:1000 diluted).Predicted band size:33.8KDa.Observed band size:38KDa.
Western blot analysis using SYP mouse mAb against rat brain tissue lysate.
Immunohistochemical analysis of paraffin-embedded human Small Intestine, myenteric plexus tissues using anti-SYP mouse mAb
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Background
Swiss-Prot Acc.P08247.Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore. Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases. Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"