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Product Name
Anti-SUZ12 (2B12) Mouse antibody
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Description
SUZ12 (2B12) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human SUZ12 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Immunofluorescence analysis of MCF-7 cells using SUZ12 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot detection of SUZ12 in Jurkat,A549 and MCF7 cell lysates using SUZ12 mouse mAb (1:1000 diluted).Predicted band size:83KDa.Observed band size:83KDa.
Immunofluorescence analysis of U251 cells using SUZ12 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Background
Swiss-Prot Acc.Q15022.This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. (Provided by RefSeq) SUZ12 is overexpressed in several human tumors, including tumors of the colon, breast and liver.Tissue specificity: Overexpressed in breast and colon cancer.
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