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Product Name
Anti-SULT2B1 antibody
- Documents
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Description
Rabbit polyclonal to SULT2B1
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Tested applications
ELISA, WB
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Species reactivity
Human SULT2B1 / HSST2
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Alternative names
AI326997 antibody; BB173635 antibody; HSST2 antibody; ST2B1 antibody; ST2B1 antibody; SULT2B antibody; Sult2b1 antibody; SULT2B1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human SULT2B1 (rh SULT2B1; NP_004596.2; Asp 2-Ser 365). SULT2B1 specific IgG was purified by human SULT2B1 affinity chromatography
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-30 μg/mL
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human SULT1B1. The detection limit for Human SULT1B1 is approximately 0.00245 ng/well.
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Validations
SULT2B1 / HSST2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
Sulfotransferase family cytosolic 2B member 1, also known as Sulfotransferase 2B1, ST2B1, Alcohol sulfotransferase, Hydroxysteroid sulfotransferase 2, SULT2B1 and HSST2, is a cytoplasm protein which belongs to the sulfotransferase 1 family. The human hydroxysteroid sulfotransferase (SULT) family is comprised of two subfamilies, SULT2A1 and SULT2B1. SULT2B1 is expressed highly in placenta, prostate and trachea. A lesser expression of SULT1B1 was observed in the small intestine and lung. SULT2B1 catalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. SULT2B1 sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol. The two SULT2B1 isoforms, SULT2B1a and SULT2B1b, are encoded by a single gene as a result of alternative transcription initiation and alternative splicing. SULT2B1b catalyzes the sulfonation of 3beta-hydroxysteroid hormones and cholesterol, whereas SULT2B1a preferentially catalyzes pregnenolone sulfonation.
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References
- Fujita K. et al., 1997, J. Biochem. 122:1052-61.
- Geese,WJ. et al., 2001,Biochem Biophys Res Commun.288 (1): 280-9.
- Shimizu,C. et al., 2003, Endocrinology. 144 (4):1186-93.
- Ji,Y. et al., 2007, J Pharmacol Exp Ther. 322 (2): 529-40.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"