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Product Name
Anti-ST18 antibody
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Description
Rabbit polyclonal antibody to ST18
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Tested applications
WB, FC
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Species reactivity
Human, Mouse, Rat, Dog, Horse, Sheep
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Alternative names
NZF3 antibody; NZF-3 antibody; ZNF387 antibody; ZC2H2C3 antibody; ZC2HC10 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human st18/znf387 451-550/1047
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
FC:3ug/Test
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Validations
Fig1: Sample:; U87MG(Human) Cell Lysate at 30 ug; Primary: Anti- ST18 at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 115 kD; Observed band size: 115 kD
Fig2: Blank control: Mouse kidney.; Primary Antibody (green line): Rabbit Anti-ST18 antibody ; Dilution: 3μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody : Goat anti-rabbit IgG-PE; Dilution: 1μg /test.; Protocol; The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"