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Anti-Spp1 antibody

Cat.#: 175006

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-Spp1 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Spp1
  • Tested applications
    WB
  • Species reactivity
    Human, Rat, Mouse
  • Alternative names
    B antibody; R antibody; Ap antibody; OP antibody; Sp antibody; mi antibody; 2AR antibody; Bsp antibody; Eta antibody; Opn antibody; Ric antibody; ost antibody; BNSP antibody; BSPI antibody; ETA- antibody; Opnl antibody; Apl-1 antibody; ETA-1 antibody; Spp-1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within c-terminal mouse osteopontin.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2000

  • Validations

    Fig1:; Western blot analysis of Osteopontin on mouse liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig1:; Western blot analysis of Osteopontin on mouse liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2:; Western blot analysis of Osteopontin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/2000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: Rat kidney tissue lysate; Lane 2: Mouse kidney tissue lysate; Lane 3: HepG2 cell lysate

    Fig2:; Western blot analysis of Osteopontin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/2000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: Rat kidney tissue lysate; Lane 2: Mouse kidney tissue lysate; Lane 3: HepG2 cell lysate

  • Background
    P10923
  • References
    • Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;Large-scale phosphorylation analysis of mouse liver.;Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
    • Ashkar S., Weber G.F., Panoutsakopoulou V., Sanchirico M.E., Jansson M., Zawaideh S., Rittling S.R., Denhardt D.T., Glimcher M.J., Cantor H.;Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity.;Science 287:860-864(2000).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"