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Product Name
Anti-SMYD3 Rabbit antibody
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Description
SMYD3 Rabbit monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
KMT3E; ZMYND1; ZNFN3A1; bA74P14.1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human SMYD3
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/2000
IHC: 1/20
ICC/IF: 1/50
FC: 1/20
IP: 1/20
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Validations
Western blot detection of SMYD3 in Jurkat,Hela cell lysates using SMYD3 Rabbit mAb(1:1000 diluted).Predicted band size:49kDa.Observed band size:49kDa.
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Background
Swiss-Prot Acc.Q9H7B4.Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed:15235609, PubMed:22419068). Also methylates 'Lys-5' of histone H4 (PubMed:22419068). Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed:15235609). Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences (PubMed:15235609).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"