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Product Name
Anti-SMN/Gemin 1 (3D11) Mouse antibody
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Description
SMN/Gemin 1 (3D11) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP
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Species reactivity
Human, Monkey
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human SMN1 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Western blot detection of SMN1 in Jurkat,K562,3T3 and Hela cell lysates using SMN1 mouse mAb (1:3000 diluted).Predicted band size:39KDa.Observed band size:35KDa.
Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and testis tissues (right) using SMN1 mouse mAb with DAB staining.
Immunofluorescence analysis of HepG2 cells using SMN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis of paraffin-embedded stomach cancer tissues (left) and brain tumor (right) using SMN1 mouse mAb with DAB staining.
Immunoprecipitation analysis of Hela cell lysates using SMN1 mouse mAb.
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Background
Swiss-Prot Acc.Q16637.This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The telomeric and centromeric copies of this gene are nearly identical and encode the same protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"