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Product Name
Anti-SMC1A (4C5) Mouse antibody
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Description
SMC1A (4C5) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Alternative names
Chromosome segregation protein SmcB;DXS423E;KIAA0178;MGC138332;Sb1.8;Segregation of mitotic chromosomes 1;SMC protein 1A;SMC-1-alpha;SMC-1A;SMC1 (structural maintenance of chromosomes 1 yeast) like 1;SMC1;SMC1 structural maintenance of chromosomes 1 like antibody
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant human SMC1A(C-term.) protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/100
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Validations
Western blot detection of SMC1A(C-term) in MOLT-4,U87 MG and HelaNE cell lysates using SMC1A (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 143KDa.Observed band size: 143KDa.
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-SMC1A mouse mAb(dilution 1:100).
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Background
Swiss-Prot Acc.Q14683.Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair . ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint. SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment.
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