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Product Name
Anti-SLAMF8 antibody
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Description
Rabbit polyclonal to SLAMF8
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Tested applications
ELISA, WB, IHC-P
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Species reactivity
Mouse SLAMF8
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Alternative names
BLAME antibody; CD353 antibody; SBBI42 antibody; Blame antibody; SBBI42 antibody; 5830408F06Rik antibody; 5830408F06Rik antibody; Blame antibody; BLAME antibody; FLJ20442 antibody; MGC129578 antibody; RP11-190A12.3 antibody; SBBI42 antibody; SBBI42 antibody; SLAMF8 antibody; SLAMF8 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse SLAMF8 (rMouse SLAMF8; NP_083360.2; Met 1-Asp 231). SLAMF8 specific IgG was purified by Mouse SLAMF8 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse SLAMF8. The detection limit for Mouse SLAMF8 is approximately 0.00245 ng/well.
IHC-P: 0.1-1 μg/mL
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Validations
SLAMF8 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
SLAMF8 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse SLAMF8 in mouse spleen with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
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Background
The signaling lymphocyte activation molecule (SLAM) family includes homophilic and heterophilic receptors that modulate both adaptive and innate immune responses. These receptors share a common ectodomain organization: a membrane-proximal immunoglobulin constant domain and a membrane-distal immunoglobulin variable domain that is responsible for ligand recognition. SLAM family of receptors is expressed by a wide range of immune cells. Through their cytoplasmic domain, SLAM family receptors associate with SLAM-associated protein (SAP)-related molecules, a group of cytoplasmic adaptors composed almost exclusively of an SRC homology 2 domain. SLAM family receptors, in association with SAP family adaptors, have crucial roles during normal immune reactions in innate and adaptive immune cells. Mouse SLAM family member 8, also known as B-lymphocyte activator macrophage expressed, BCM-like membrane protein, SLAMF8 and BLAME, is a single-pass type I membrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain. SLAMF8 / BLAME is expressed in lymph node, spleen, thymus and bone marrow. It may play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor.
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References
- Kingsbury G.A., et al., 2001, J. Immunol. 166:5675-80.
- Zhang Z., et al., 2004, Protein Sci. 13: 2819-24.
- Veillette,A. et al., 2006, Nat Rev Immunol 6 (1): 56-66.
- Veillette,A. et al., 2006, Trends Immunol 27 (5): 228-34.
- Yan,Q. et al., 2007, Proc Natl Acad Sci. USA.104 (25):10583-8.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"