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Product Name
Anti-SIRT2 (1D4) Mouse antibody
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Description
SIRT2 (1D4) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
FLJ35621;FLJ37491;NAD dependent deacetylase sirtuin 2;NAD-dependent deacetylase sirtuin-2;NAD-dependent protein deacetylase sirtuin-2;Regulatory protein SIR2 homolog 2;Silencing information regulator 2 like;Silent information regulator 2;Silent mating typ antibody
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Isotype
Mouse IgG2b
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Preparation
Antigen: Purified recombinant human SirT2 protein expressed in E.coli.
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Clonality
Monoclonal
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Formulation
PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
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Validations
Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20u2103 and using SirT2 (168470,dilution 1:50) mouse mAb (green) and Histone H3.1 (Phospho-Ser10) (310045,dilution 1:200) Rabbit pAb (red).
Western blot detection of SirT2 in PC-12, Rat Brain and Mouse Brain cell lysates using SirT2 mouse mAb (1:1000 diluted). Predicted band size: 39,43KDa. Observed band size:39,43KDa.
Western blot detection of SirT2 in Rat Cerebral Cortex and Rat Brain lysates using SirT2 mouse mAb (1:1000 diluted). Predicted band size: 39,43KDa. Observed band size:39,43KDa.
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Background
Swiss-Prot Acc.Q8IXJ6.Participate in a variety of cellular functions, including histone deacetylation, gene silencing, chromosomal stability, and aging. SIRT2, a human homolog of the yeast SIR2, functions as transcriptional silencing mediator at mating-type loci, telomeres and ribosomal gene clusters. SIRT2 expression increases dramatically during mitosis and is multiply phosphorylated at the G(2)/M transition of the cell cycle.
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