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Product Name
Anti-sialate O-acetylesterase antibody
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Description
Rabbit polyclonal to sialate O-acetylesterase
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Tested applications
ELISA, IHC-P
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Species reactivity
Human SIAE / AIS6
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Alternative names
AIS6 antibody; clone 165 antibody; CSEC antibody; CSE-C antibody; cytosolic sialic acid 9-O-acetylesterase homolog antibody; H-Lse antibody; LSE antibody; LSE antibody; MGC87009 antibody; sialate O-acetylesterase antibody; sialate O-acetylesterase antibody; sialic acid-specific 9-O-acetylesterase antibody; sialic acid-specific acetylesterase II antibody; yolk sac gene 2 antibody; yolk sac protein 2 antibody; YSG2 antibody; Ysg2 antibody; LSE antibody; AIS6 antibody; CSEC antibody; YSG2 antibody; CSE-C antibody; LSE antibody; Ysg2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human SIAE / AIS6 (rh SIAE / AIS6; Q9HAT2-1; Met1-Lys523). SIAE / AIS6 specific IgG was purified by Human SIAE / AIS6 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SIAE / AIS6. The detection limit for Human SIAE / AIS6 is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
sialate O-acetylesterase / SIAE Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human SIAE in human colon with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
sialate O-acetylesterase / SIAE Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human SIAE in human prostate with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Sialate O-acetylesterase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. It is widely expressed with high expression in the testis, prostate, and colon. The systematic name of this enzyme class is N-acyl-O-acetylneuraminate O-acetylhydrolase. Other names in common use include N-acetylneuraminate acetyltransferase, sialate 9(4)-O-acetylesterase, and sialidase. Sialate O-acetylesterase catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. Defects in Sialate O-acetylesterase are a cause of autoimmune disease type 6 (AIS6). Individuals manifesting susceptibility to autoimmune disease type 6 can suffer from juvenile idiopathic arthritis, rheumatoid arthritis, multiple sclerosis, Sjogren syndrome, systemic lupus erythematosus, type 1 diabetes, ulcerative colitis, and Crohn disease.
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References
- Mandal C, et al. (2012) Regulation of O-acetylation of sialic acids by sialate-O-acetyltransferase and sialate-O-acetylesterase activities in childhood acute lymphoblastic leukemia. Glycobiology. 22(1): 70-83.
- Tsai S, et al. (2011) Transcriptional profiling of human placentas from pregnancies complicated by preeclampsia reveals disregulation of sialic acid acetylesterase and immune signalling pathways. Placenta. 32 (2): 175-82.
- Surolia I, et al. (2010) Functionally defective germline variants of sialic acid acetylesterase in autoimmunity. Nature. 466 (7303): 243-7.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"