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Anti-SHH antibody

Cat.#: 176520

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Product Information

  • Product Name
    Anti-SHH antibody
  • Documents
  • Description
    Rabbit monoclonal antibody to SHH
  • Tested applications
    WB, ICC/IF, IHC-P, FC
  • Species reactivity
    Human
  • Alternative names
    TPT antibody; HHG1 antibody; HLP3 antibody; HPE3 antibody; SMMCI antibody; ShhNC antibody; TPTPS antibody; MCOPCB5 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:1,000-1:2,000

    ICC/IF: 1:50-1:200

    IHC-P: 1:200-1:500

    FC: 1:50-1:100

  • Validations

    Fig1:; Western blot analysis of Sonic Hedgehog Protein on different lysates using anti-Sonic Hedgehog Protein antibody at 1/1,000 dilution.; Positive control:; Lane 1: Hela; Lane 2: HepG2

    Fig1:; Western blot analysis of Sonic Hedgehog Protein on different lysates using anti-Sonic Hedgehog Protein antibody at 1/1,000 dilution.; Positive control:; Lane 1: Hela; Lane 2: HepG2

    Fig2:; ICC staining Sonic Hedgehog Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2:; ICC staining Sonic Hedgehog Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3:; ICC staining Sonic Hedgehog Protein in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3:; ICC staining Sonic Hedgehog Protein in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4:; ICC staining Sonic Hedgehog Protein in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4:; ICC staining Sonic Hedgehog Protein in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5:; Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.

    Fig5:; Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.

    Fig6:; Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.

    Fig6:; Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.

    Fig7:; Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.

    Fig7:; Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.

    Fig8:; Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.

    Fig8:; Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.

    Fig9:; Flow cytometric analysis of Hela cells with Sonic Hedgehog Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

    Fig9:; Flow cytometric analysis of Hela cells with Sonic Hedgehog Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

  • Background
    Q15465
  • References
    • Zhang X et al. Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up. Mod Pathol 28:654-61 (2015).
    • Li H et al. Olfactomedin 4 deficiency promotes prostate neoplastic progression and is associated with upregulation of the hedgehog-signaling pathway. Sci Rep 5:16974 (2015).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"