Anti-Sele antibody

Cat.#: 175406

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Product Information

  • Product Name
    Anti-Sele antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Sele
  • Tested applications
    WB, IHC-P
  • Species reactivity
    Human, Mouse, Rat, Chicken, Dog, Pig, Horse, Rabbit
  • Alternative names
    El antibody; Elam antibody; CD62E antibody; E-sele antibody; ELAM-1 antibody; LECAM2 antibody; E-selectin antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from mouse e-selectin 501-612/612
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    IHC-P:1:400-800

  • Validations

    Fig1: Sample: HL-60 Cell (Human) Lysate at 40 ug; Primary: Anti- E-Selectin at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 65 kD; Observed band size: 65 kD

    Fig1: Sample: HL-60 Cell (Human) Lysate at 40 ug; Primary: Anti- E-Selectin at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 65 kD; Observed band size: 65 kD

    Fig2: Sample: Liver (Mouse) Lysate at 40 ug; Primary: Anti- E-Selectin at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 65 kD; Observed band size: 65 kD

    Fig2: Sample: Liver (Mouse) Lysate at 40 ug; Primary: Anti- E-Selectin at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 65 kD; Observed band size: 65 kD

    Fig3: Sample:; DU145 Cell Lysate at 40 ug; LOVO Cell Lysate at 40 ug; Primary: Anti-E-Selectin (wt-p53) at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 65 kD; Observed band size: 63 kD

    Fig3: Sample:; DU145 Cell Lysate at 40 ug; LOVO Cell Lysate at 40 ug; Primary: Anti-E-Selectin (wt-p53) at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 65 kD; Observed band size: 63 kD

    Fig4: Sample: Siha Cell Lysate at 40 ug; Primary: Anti- E-Selectin at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 65 kD; Observed band size: 65 kD

    Fig4: Sample: Siha Cell Lysate at 40 ug; Primary: Anti- E-Selectin at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 65 kD; Observed band size: 65 kD

  • Background

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"