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Product Name
Anti-Sele antibody
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Description
Rabbit polyclonal antibody to Sele
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat, Chicken, Dog, Pig, Horse, Rabbit
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Alternative names
El antibody; Elam antibody; CD62E antibody; E-sele antibody; ELAM-1 antibody; LECAM2 antibody; E-selectin antibody
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Isotype
IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from mouse e-selectin 501-612/612
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
IHC-P:1:400-800
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Validations
Fig1: Sample: HL-60 Cell (Human) Lysate at 40 ug; Primary: Anti- E-Selectin at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 65 kD; Observed band size: 65 kD
Fig2: Sample: Liver (Mouse) Lysate at 40 ug; Primary: Anti- E-Selectin at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 65 kD; Observed band size: 65 kD
Fig3: Sample:; DU145 Cell Lysate at 40 ug; LOVO Cell Lysate at 40 ug; Primary: Anti-E-Selectin (wt-p53) at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 65 kD; Observed band size: 63 kD
Fig4: Sample: Siha Cell Lysate at 40 ug; Primary: Anti- E-Selectin at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 65 kD; Observed band size: 65 kD
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"